Our Team

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Dr Nicky O’Boyle

Principal Investigator

Dr Nicky O’Boyle is an Assistant Professor of Microbiology at Trinity College Dublin. He has been working in the academic research sector since 2008 and has published more than 20 research articles in leading life science journals. His work aims to improve understanding of why and how bacteria make us sick, using this knowledge to make better treatments for infections. His PhD with Dr Aoife Boyd at the University of Galway focused on investigating how Vibrio parahaemolyticus – a leading global cause of shellfish related food poisoning – attaches to the cells of the intestine. He then moved to The University of Glasgow in 2014 where he worked in the laboratory of Dr Robert Davies, developing advanced airway cell culture models to study infection dynamics of the livestock pathogen Mannheimia haemolytica. In 2017, at The University of Glasgow, Dr O’Boyle joined Professor Andrew Roe’s Group where he studied diverse strains of Escherichia coli associated with distinct disease outcomes in humans. His independent research group was established in 2022 at University College Cork before relocating to Trinity College Dublin in 2024. Their work encompasses investigating the fundamental molecular biology of bacterial pathogens, and the development of novel therapeutic strategies to combat bacterial disease. Dr O’Boyle’s group have a strong focus on Gram-negative enteric pathogens including enterohaemorrhagic E. coli, Crohn’s disease-associated pathobionts, and uropathogenic E. coli. His group aim to exploit insights into niche-specific pathogen expression patterns to develop precision anti-virulence strategies.

Dr Margot Fargeas

Postdoctoral Researcher

Margot completed her entire academic training at Université Clermont Auvergne (France), where she developed a strong interest in host–pathogen interactions and their impact on human health. At the end of her Master’s degree, she was awarded a competitive national fellowship to pursue a PhD under the supervision of Pr. Nicolas Barnich and Pr. Anthony Buisson within the M2iSH laboratory. Her doctoral research focused on the characterization of a virulence factor in adherent-invasive Escherichia coli (AIEC) associated with Crohn’s disease. This work opened new perspectives for the development of inhibitory strategies aimed at reducing the virulence of these pathobionts. After completing her PhD in December 2024, she joined the research group of Dr. Nicky O’Boyle at Trinity College Dublin as part of a project funded by the HRB Emerging Investigator Award. Within this team, her work involves developing patient-derived organoids generated from biopsies of individuals with Crohn’s disease to investigate the virulence of Enterobacteriaceae strains isolated from these patients. The project also explores the impact of D-amino acids on the pathogenicity of these bacteria during infection.

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Weronika Leśniak

PhD Student

Weronika completed a PLC course in Laboratory Techniques in Killester College of Further Education in 2020. In 2024, she completed her undergraduate degree in Microbiology in Trinity College Dublin. She pursued a MScR in Trinity College under the supervision of Professor Marta Martins. Her work focused on uncovering potential alternatives to antibiotics to treat Pseudomonas aeruginosa infections in Cystic Fibrosis patients. Weronika started her PhD in 2025 and is working on bioprospecting marine sponge-associated bacteria for novel anti-virulence therapeutics. This project is supervised by Dr Nicky O’Boyle and Dr Fabio Boylan based in TBSI.

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Saoirse Flavin

PhD Student

Saoirse completed her undergraduate degree in Microbiology in University College Cork in 2025. She started her PhD in 2025, and is working on assessing the potential of D-amino acids to act as therapeutics in Crohn’s disease. Her research interests focus on elucidating the mechanistic basis underlying infectious disease and modulating interactions between pathogens and their host. Saoirse is particularly interested in understanding how D-amino acids regulate the expression of specific virulence factors in E. coli and exploring their potential as novel therapeutic agents for clinically significant infections. 

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